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Evaluation of Transforaminal Endoscopic Discectomy in
The degenerative nucleus pulposus was extracted by proprietary nucleus pulposus forceps through the intervertebral foramen. Clearly visible dura under endoscopy and dura with the heart beat were the significant signs for thoroughly decompressing and terminating the surgery (Figure 4). The negative result of straight-leg raising test further .
Lasers in percutaneous disc surgery
which is visible (‘green’) with a wavelength of 532 nm. Their has been mainly clinical experience with this laser-type in various surgical specialties mainly for cutting and coagulating tissue (45). The absorption characteristics are shown in Table 3. Since there is no absorption peak of nucleus pulposus at …
The responses of nucleus pulposus cells to pressure and
Intervertebral disc is composed of nucleus pulposus (NP), anulus fibrosus (AF), and vertebral endplate. The biomechanical functions of the disc include the support of body weight and absorption of …
(PDF) Primary immune system responders to nucleus pulposus
The disc space is opened with anulus trephines and the nucleus pulposus is removed with rigid and flexible forceps as well as with automated shaver systems under intermittent endoscopic control .
Up-Regulation of Pain Behavior and Glial Activity in the
1. Asian Spine J. 2014 Oct;8(5):549-56. doi: 10.4184/asj.2014.8.5.549. Epub 2014 Oct 18. Up-Regulation of Pain Behavior and Glial Activity in the Spinal Cord after Compression and Application of Nucleus Pulposus onto the Sciatic Nerve in Rats.
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Normoxic stabilization of HIF-1α drives glycolytic
The nucleus pulposus is an aggrecan-rich, avascular tissue that permits the intervertebral disk to resist compressive loads. In the disk, nucleus pulposus cells express hypoxia-inducible factor (HIF)-1α, a transcription factor that responds to oxygen tension and regulates glycolysis. The goal of the present study was to examine the importance of HIF-1α in rat nucleus pulposus cells and to probe the function …
Normoxic stabilization of HIF-1α drives glycolytic
Cell culture in hypoxia. Nucleus pulposus cells were cultured in an Hypoxia Work Station (Invivo 2 300; Ruskinn, Brigend, UK) with a mixture of 2% O 2, 5% CO 2, and 93% N 2 for 24–72 h. The concentration of oxygen chosen for this study was selected on the basis of our previous in vitro studies and information on the oxemic status of the disk in vivo.
(PDF) Hydrated nucleus pulposus extrusion in dogs
Hydrated nucleus pulposus extrusions (HNPE), previously misinterpreted as discal cysts (Konar et al., 2008;Kamishina et al., 2010), are a type of disc herniation in which non-degenerated or .
An optimized step-by-step protocol for isolation of
An optimized step-by-step protocol for isolation of nucleus pulposus, annulus fibrosus, and end plate cells from the mouse . shows the major components of the disc; SafO/Fast Green, D, staining shows the relative distribution of proteoglycan (stained orange-red) and collagen (stained fast green). . † Serrated standard pattern forceps .
Percutaneous endoscopic lumbar discectomy (PELD)
The disc space is opened with anulus trephines and the nucleus pulposus is removed with rigid and flexible forceps as well as with automated shaver systems under intermittent endoscopic control (discoscopy). The procedure is performed in local anesthesia. The results of the first thirty patients with a follow-up time between 6 months and 17 .
Extracellular Matrix Ligand and Stiffness Modulate
Extracellular Matrix Ligand and Stiffness Modulate Immature Nucleus Pulposus Cell-Cell Interactions Christopher L. Gilchrist1, Eric M. Darling2,3,4, Jun Chen5, Lori A. Setton1,5* 1Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States of America, 2Department of Molecular Pharmacology, Physiology, and
Transforaminal Endoscopic Forceps – TITMED
Number: Description: Image : A6001: Nucleus pulposus forceps (Φ2.5x320mm) A6002 : Nucleus pulposus forceps (Φ2.5x320mm) A6022: Biopsy forceps (Φ3x330mm)
Comparison of the application value of two commonly used
or protruding nucleus pulposus were removed by nucleus pulposus forceps, and the nerve root canal was expanded. The conventional intervertebral foramen forming treatment was performed. After the nerve root was loosened satisfactorily, the wound was disinfected and treated to stop bleeding, the cannula was withdrawn, and the incision was sutured.
ANTI-HMGB1 NEUTRALIZING ANTIBODY IMPROVES PAIN
pulposus The rats were placed in the prone position, and an incision was made in the middle of the proximal tail (n=3). NP was harvested from the disc of coccygeal spine by the forceps and froze in liquid nitrogen. Immunoblotting of naive NP was performed. Experiment 2:HMGB1 involvement in neuropathic pain induced by nucleus pulposus application
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Upregulation of BNIP3 and translocation to mitochondria in
· Nucleus pulposus cells were grown on poly-L-lysine-coated coverslips and after treatment, cells were labeled with 500 nM Mitotracker (Invitrogen, USA) for 45 min at 37 °C. After washing three times with PBS, cells were fixed for 10 min with 4% paraformaldehyde and then permeabilized with 0.2% Triton X-100 for 30 min at room temperature.
Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells
Tauroursodeoxycholic acid (TUDCA) is a kind of hydrophilic bile acid, which could protect cells from death via inhibiting endoplasmic reticulum (ER) stress. However, the role of TUDCA in compression-induced intervertebral disc degeneration (IVDD) has not been elucidated. Here, we used a previously described device to mimic in vivo > compression conditions.
(PDF) Calcification of intervertebral discs in the
Calcium deposits (arrows) in the nucleus pulposus (Np) and in a broad discontinuous ring within the annulus fibrosus (Af): 'severe' degree of calcification (3). Von Kossa; Bar = 1.6 mm. Figures .
Polyurethane scaffold with in situ swelling capacity for
· Nucleus pulposus cells (NPCs) were isolated from 4 to 10 months old bovine caudal IVD and cultured with DMEM, 1% Pen/Strep, 10% fetal calf serum (FCS, Gibco) at 37 °C under normoxic condition . Human bone marrow aspirates were obtained with ethical approval from the Swiss cantonal authorities (KEK 188/10) and informed consent.
10.1016/j.jbspin.2011.04.011 | DeepDyve
· The apoptosis of the nucleus pulposus cell appears as a result caused by aging and progressive disc deterioration, which in turn, the cell death accelerates the disc aging and degeneration [28,29] . However, the exact mechanism of nucleus pulposus cell death is still unknown.
BNIP3 is a member of the BH3-only subfamily of Bcl-2 proteins.
MAXDISC® Endoscopic System - MaxMore
Nucleus Discectomy – Grasping forceps are used to remove excess nucleus material. 2. Nucleus Ablation – By using our bipolar RF probe a safe ablation of the nucleus can be performed. 3. Annulus Modulation – By using our flexible RF probe, pain receptors can be effectively treated because the energy can be guided to the annular wall. 4.
The Evolution and Advancement of Endoscopic Foraminal
The absorption is significantly enhanced by the presence of pigments such as hemoglobin or melanin. 5 Absorption also increases in presence of indigo carmine; a blue-green vital dye, which is taken preferentially by the degenerative nucleus pulposus while normal tissues remain relatively unstained. 5 Removal of stained degenerative disc tissue .